human ox ldl  (MedChemExpress)

Journal: Environment & Health

Article Title: Brominated Flame Retardants Exposure Promotes the Formation of Atherosclerotic Plaque in Aortic Roots of ApoE –/– Mice by Increasing Macrophage Recruitment and Foam Cell Formation

doi: 10.1021/envhealth.4c00183

Figure Lengend Snippet: BFRs promote the formation of macrophage-derived foam cells. (A) THP-1 macrophages were treated with different concentrations of BDE-47, BDE-209 and DBDPE for 24 h, and then the cell viability was detected by CCK-8 assay. Data are expressed as mean ± SD, n = 4, *** P < 0.001, vs Control. (B) Statistical graph of intracellular lipid content detected by oil red O staining. THP-1 macrophages were pre-exposed to different concertrations of BFRs for 24 h, then incubated with 50 μg/mL Ox-LDL for an additional 24 h. Data are expressed as mean ± SD, n = 3, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 vs Control; (C) Representative pictures of oil red O staining.

Article Snippet: THP-1 macrophages were pretreated with or without BFRs (the final concentration of DMSO was 0.1%) for 24 h and then incubated with 50 μg/mL human oxidized low density lipoprotein (Ox-LDL, Yeasen, China) for an additional 24 h at 37 °C.

Techniques: Derivative Assay, CCK-8 Assay, Control, Staining, Incubation